Development of Novel Cytoplasmic Male Sterile Source from Dongxiang Wild Rice (Oryza rufipogon)

doi:10.1016/S1672-6308(13)60145-6

Abstract

Abstract:

This study was conducted to develop and characterize a novel cytoplasmic male sterile (CMS) source which was identified from Dongxiang wild rice (Oryza rufipogon) by crossing Dongxiang wild rice as female with Zhongzao 35, an indica inbred variety, as male and continuous backcrossing with Zhongzao 35. Observation under optical microscope manifested that this novel CMS belonged to typical abortion type with less pollen compared with wild abortive type cytoplasm (CMS-WA). Sequential planting showed that this novel CMS has complete and stable male sterility. Testcross experiment showed that all the 24 tested materials including maintainer and restorer lines of CMS-WA and Honglian type cytoplasm (CMS-HL) and other indica inbred varieties are the maintainers with complete maintaining ability, suggesting that this novel CMS has fertility restoration totally different from CMS-WA and CMS-HL and belongs to a novel type of CMS. So far, we only discovered a unique fertility restoration source for this novel CMS. Inheritance analysis showed that the fertility restoration of this CMS was governed by three pairs of independent dominant genes. Prospect for application of this novel CMS system in hybrid rice breeding was also discussed.

Key words: Dongxiang wild rice, cytoplasmic male sterility, fertility restoration

SHEN Xian-hua, YAN Song, HUANG Ren-liang, ZHU Shan, XIONG Hong-liang, SHEN Lin-jun. Development of Novel Cytoplasmic Male Sterile Source from Dongxiang Wild Rice (Oryza rufipogon)[J]. RICE SCIENCE, 2013, 20(5): 379-382.

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